Phage therapy as an approach to control salmonella enterica serotype enteritidis infection in mice
Rev. Soc. Bras. Med. Trop
; 52: e20190290, 2019. graf
Artigo
em Inglês
| LILACS
| ID: biblio-1057257
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
Salmonella enterica serotype Enteritidis (S. Enteritidis) is a cause of food-borne human illness. Given the prevalence of antibiotic resistance of Salmonella Enteritidis and the lack of antibiotic efficacy in future years, its replacement with other agents is necessary. One of the most useful agents is bacteriophages. METHODS S. Enteritidis was identified using a multiplex polymerase chain reaction assay. The effective bacteriophages were isolated from hospital wastewater samples. The effects of the bacteriophages were evaluated both in vitro and in vivo. RESULTS The phage SE20 belonged to the Podoviridae family, and the genome size was 40 kb. The evaluation of phage SE20 at variable pH ranges showed its susceptibility to pH < 3 and pH > 12. The animal model showed that mice infected with S. Enteritidis developed hepatomegaly and splenomegaly, but did not experience gastrointestinal complications after receiving the bacteriophages. CONCLUSIONS The results of this study suggest that phage SE20 is a promising candidate for controlling salmonellosis caused by Salmonella Enteritidis.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
/
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Doenças Negligenciadas
/
Zoonoses
Base de dados:
LILACS
Assunto principal:
Salmonella enteritidis
/
Infecções por Salmonella
/
Terapia por Fagos
Tipo de estudo:
Estudo prognóstico
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Irã
Instituição/País de afiliação:
School of Public Health (TUMS)/IR
/
Tehran University of Medical Sciences (TUMS)/IR
/
Tehran University of Medical Sciences/IR