Expression and characterization of HlyX hemolysin from Leptospira interrogans serovar Copenhageni Potentiation of hemolytic activity by LipL32
Biochem. biophys. res. commun
; 333(4): 1341-1347, 2005.
Article
em En
| SES-SP, SESSP-IBPROD, SES-SP, SESSP-IBACERVO
| ID: biblio-1060801
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
The HlyX, a putative hemolysin identified from the Leptospira genomes, was cloned, expressed in Escherichia coli, purified, and its hemolytic activity was confirmed. Mouse polyclonal antiserum against the recombinant HlyX recognized HlyX-related antigens in a panel of Leptospira species extracts and it was also able to abolish the hemolytic activity of HlyX. A mixture of HlyX and LipL32, a known hemolysin from Leptospira, induced hemolysis in a synergistic way that was fully inhibited by antiserum against either protein. Moreover, sera from patients with leptospirosis also recognized the recombinant HlyX, showing that it is presented to the host immune system during Leptospira infection.
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Coleções:
06-national
/
BR
Base de dados:
SES-SP
/
SESSP-IBACERVO
/
SESSP-IBPROD
Assunto principal:
Proteínas de Escherichia coli
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Escherichia coli
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Leptospira interrogans
Limite:
Animals
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Female
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Humans
Idioma:
En
Revista:
Biochem. biophys. res. commun
Ano de publicação:
2005
Tipo de documento:
Article