Modulation of miR-26a-5p and miR-15b-5p exosomal expression associated with Clopidogrel-induced hepatotoxicity in HepG2 Cells
Front. pharmacol
; 12(8): 906-906, 2017.
Artigo
em Inglês
| Sec. Est. Saúde SP, SESSP-IDPCPROD, Sec. Est. Saúde SP
| ID: biblio-1062901
Biblioteca responsável:
BR79.1
Localização: BR79.1
ABSTRACT
Clopidogrel is an essential antiplatelet drug used to prevent thrombosis complications associated with atherosclerosis. However, hepatotoxicity is a potential adverse effect related to clopidogrel therapy. Exosome-derived miRNAs may be useful for improved monitoring of drug response and hepatotoxicity risk. In the present study, the expression of several exosomal miRNAs (miR-26a-5p, miR-145-5p, miR-15b-5p, and miR-4701-3p) and cell-derived mRNA targets (PLOD2, SENP5, EIF4G2, HMGA2, STRADB, and TLK1) were evaluated in HepG2 cells treated with clopidogrel (6.25, 12.5, 25, 50, and 100 μM) for 24 and 48 h. Then, clopidogrel cytotoxicity was evaluated by analyzing DNA fragmentation and the cell cycle profile using flow cytometry. Differential expression of exosome-derived miRNAs and cell-derived mRNAs was analyzed by RT-qPCR. Exposure of HepG2 cells to high concentrations of clopidogrel (50 and 100 μM) for 24 h caused significant DNA fragmentation (17.6 and 44.4%, respectively; p < 0.05) and 48 h (26.8 and 48.9%, respectively; p < 0.05), indicating cellular toxicity...
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Coleções:
Bases de dados nacionais
/
Brasil
Base de dados:
Sec. Est. Saúde SP
/
SESSP-IDPCPROD
Assunto principal:
Trombose das Artérias Carótidas
/
Linhagem Celular
/
MicroRNAs
Tipo de estudo:
Fatores de risco
Idioma:
Inglês
Revista:
Front. pharmacol
Ano de publicação:
2017
Tipo de documento:
Artigo
Instituição/País de afiliação:
Federal University of Rio Grande do Norte/BR
/
Instituto dante Pazzanese de Cardiologia/BR
/
São Paulo State University (UNESP)/BR
/
University of Campinas/BR
/
University of São Paulo/BR