Galanin-like immunoreactivity in the brain of the snake Bothrops jararaca

ABSTRACT

The distribution of galanin-like immunoreactive perikarya and nerve fibers in the brain of the snake Bothrops jararaca was studied by means of immunohistochemistry using an antiserum against porcine galanin. Immunoreactive neurons were only detected in the infundibular recess nucleus. Immunoreactive fibers were found in the telencephalic, diencephalic and mesencephalic areas such as the dorsal cortex, nucleus accumbens, lamina terminalis, preoptic area, mediodorsal region of the supraoptic nucleus, subfornical organ, nucleus of the paraventricular organ, subcommisural organ and periventricular grey region. The habenula, paraventricular nucleus, infundibular recess nucleus and hypothalamo-hypophyseal tract presented denser innervations. The outer layer of the median eminence displayed numerous fibers located close to the portal system, while scarce fibers were seen in the inner median eminence and neural lobe of the hypophysis. The distribution of labelled neurons in the brain of this snake was more restricted than that described in a turtle. The wide hypothalamic and extrahypothalamic distribution of labelled fibers suggests that galanin peptides may have hypophysiotropic, neuromodulator and neurotransmitter roles in the snake B. jararaca.