The recombinant LIC10508 is a plasma fibronectin, plasminogen, fibrinogen and C4BP-binding protein of Leptospira interrogans
Pathogens and Disease
; 74(2): 1-11, Dez, 2015. graf, ilus
Artigo
em Inglês
| Sec. Est. Saúde SP, SESSP-SUCENPROD, Sec. Est. Saúde SP
| ID: biblio-1065162
Biblioteca responsável:
BR93.2
Localização: BR93.2
ABSTRACT
Leptospirosis is a zoonosis caused by pathogenic Leptospira spp. In this study, we report that the recombinant proteins LIC10507, LIC10508 and LIC10509 are recognized by confirmed leptospirosis serum samples at both phases of the disease. The recombinant rLIC10508 and rLIC10507 are plasminogen (PLG)-binding proteins, capable of generating plasmin in the presence of a PLG activator. The proteins bind to PLG in a dose-dependent and saturable manner, fulfilling hostligandinteraction. Furthermore, rLIC10508 interacts with fibrinogen (Fg), plasma fibronectin and C4b binding protein (C4BP). The binding of rLIC10508 to Fg decreases the fibrin clotting in a thrombin-catalyzed reaction. The incubation with 4 μMof protein promoted 40% inhibition upon clotting formation. C4BP bound to rLIC10508 retained its cofactor activity forfactor I promoting the cleavage of C4b protein, which may reduce the membrane attack complex formation. Although these proteins have high amino acid sequence similarity, rLIC10508 is the most talented of the three, a behavior that might be explained by its unique putative 3D structure, whereas structures of rLIC10507 and rLIC10509 are very similar. Plasmingeneration (rLIC10507 and rLIC10508), together with decreasing fibrin clot formation (rLIC10508) and impairment of the complement system (rLIC10508) may help the bacteria to overcome host defense, facilitating the infection process...
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Base de dados:
Sec. Est. Saúde SP
/
SESSP-SUCENPROD
Assunto principal:
Fibrinolisina
/
Leptospira
/
Leptospirose
Limite:
Humanos
Idioma:
Inglês
Revista:
Pathogens and Disease
Ano de publicação:
2015
Tipo de documento:
Artigo
Instituição/País de afiliação:
Instituto Butantan/BR
/
USP/BR