Effects of myocardial infarction and an intensive exercise bout on kinetic of lactate, and LDH-B levels in spontaneously hypertensive rats
Motriz (Online)
; 26(1): e10200156, 2020. tab, graf
Artigo
em Inglês
|
LILACS-Express
| LILACS
| ID: biblio-1091248
Biblioteca responsável:
BR1.1
ABSTRACT
Aims:
This study aimed to evaluate the kinetics of lactate and lactate dehydrogenase B (LDH-B) protein levels as well as the maximum effort capacity of spontaneously hypertensive rats (SHRs) with experimental acute myocardial infarction (AMI).Methods:
thirty-two SHRs were divided into (n=8/group) S (sham), SE (sham+exercise), I (AMI), and IE (AMI+exercise). A maximum exercise test (treadmill) was evaluated before AMI or sham surgery. Echocardiography was performed 48h after the surgery. Lactacidemia was assessed at rest and during an intense exercise bout (48h after echocardiography). A two-way ANOVA followed by the post-hoc (Bonferroni) test was used, p<0.05.Results:
In the end, the heart was removed for analysis of LDH-B. AMI resulted in lower cardiac output (S vs I ∆51.3%, p<0.001), ejection fraction (S vs I ∆60.5%, p<0.001) and shortening fraction (S vs I ∆72.4%, p<0.001). The IE showed a reduction in exercise capacity when compared with pre-AMI values (1.50±0.1 vs 1.38±0.2 km/h; p=0.030) but not when compared with SE (1.41±0.3 vs 1.38±0.2 km/h; p=0.208). During the exhaustion exercise session, IE group showed lower lactacidemia at 12 min (∆9.7%, p=0.042) and 18 min (∆8.3%, p=0.038). No differences were observed in the protein level of LDH-B among the groups (p=0.573). However, when the AMI factor was considered alone, LDH-B expression was lower (sham vs AMI rats, p=0.040).Conclusion:
LDH-B protein levels in cardiac tissue appear to be associated with AMI only. Furthermore, AMI induced a reduction in exercise capacity but did not affect lactacidemia during the intense exercise bout.(AU)
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Idioma:
Inglês
Revista:
Motriz (Online)
Assunto da revista:
EducaÆo F¡sica e Treinamento
/
Medicina Esportiva
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Medicina F¡sica e ReabilitaÆo
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Fundação Universitária de Cardiologia/BR
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Universidade Federal de Ciências da Saúde de Porto Alegre/BR