Differences in Proteomic Profiles Between Caries Free and Caries Affected Children
Pesqui. bras. odontopediatria clín. integr
; 20: e5592, 2020. tab
Artigo
em Inglês
| LILACS, BBO - Odontologia
| ID: biblio-1135503
Biblioteca responsável:
BR1264.1
ABSTRACT
Abstract Objective:
To determine if there are differences in protein profiles in saliva depending if children of caries-free versus caries affected. Material andMethods:
A cohort of 91 children with ages between 6 and 19 years, along clinical status of caries experience. Protein profiles in saliva were determined using electrophoresis and the calculation of the percentage of a specific band at a specific molecular weight in relationship to the total protein in that sample (% of total) using molecular weight standards. This quantification was repeated for each protein band across a range of molecular weights for each sample. Chi-square, Fisher's exact, and Student t-tests were used to compare the distributions between caries-free and caries affected children (α=0.05).Results:
Histatin was more likely to be non-detectable or reduced in caries-free children (OR=7.56; 95% CI 1.62-35.13) and these children had on average one less gel band detected by the assay we used.Conclusion:
We have found differences in proteins between caries affected and caries-free children, suggesting that this line of investigation holds the promise of providing new tools for caries management.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
BBO - Odontologia
/
LILACS
Assunto principal:
Saliva
/
Cárie Dentária
/
Proteômica
/
Eletroforese
Tipo de estudo:
Guia de prática clínica
Limite:
Adolescente
/
Adulto
/
Criança
/
Feminino
/
Humanos
/
Masculino
País/Região como assunto:
América do Norte
Idioma:
Inglês
Revista:
Pesqui. bras. odontopediatria clín. integr
Assunto da revista:
Odontologia
Ano de publicação:
2020
Tipo de documento:
Artigo
País de afiliação:
Estados Unidos
Instituição/País de afiliação:
University of Arizona/US
/
University of Pittsburgh/US