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Increased expression of interleukin-6 gene in gastritis and gastric cancer
Santos, M P; Pereira, J N; Delabio, R W; Smith, M A C; Payão, S L M; Carneiro, L C; Barbosa, M S; Rasmussen, L T.
Afiliação
  • Santos, M P; Faculdade de Medicina de Marília. Laboratório de Genética. Marília. BR
  • Pereira, J N; Faculdade de Medicina de Marília. Laboratório de Genética. Marília. BR
  • Delabio, R W; Faculdade de Medicina de Marília. Laboratório de Genética. Marília. BR
  • Smith, M A C; Universidade Federal de São Paulo. Departamento de Morfologia. São Paulo. BR
  • Payão, S L M; Faculdade de Medicina de Marília. Laboratório de Genética. Marília. BR
  • Carneiro, L C; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia. BR
  • Barbosa, M S; Universidade Federal de Goiás. Instituto de Patologia Tropical e Saúde Pública. Goiânia. BR
  • Rasmussen, L T; Faculdade de Medicina de Marília. Laboratório de Genética. Marília. BR
Rev. bras. pesqui. méd. biol ; Braz. j. med. biol. res;54(7): e10687, 2021. tab, graf
Article em En | LILACS | ID: biblio-1249315
Biblioteca responsável: BR1.1
ABSTRACT
Helicobacter pylori (H. pylori) induces an intense inflammatory response, mediated by proinflammatory cytokines, including interleukin (IL)-6 and its membrane receptor (IL-6R), which activates important signaling pathways in the development of gastric disease and cancer. We investigated the gene and protein expression of IL-6 and IL-6R and the influence of polymorphisms rs1800795, rs1800796, and rs1800797 on its gene expression together with H. pylori infection. Furthermore, an in-silico analysis was performed to support our results. Gastric biopsies were obtained from patients with gastric symptoms and patients with gastric cancer (GC) and were divided into groups (Control, Gastritis, and Cancer). H. pylori was detected by PCR. Real-time-qPCR was employed to determine gene expression, and western blot assay was used to analyze protein expression levels. PCR-RFLP was used to characterize IL-6 polymorphisms. Bioinformatics analyses were performed using the Gene Expression Omnibus (GEO) database and GEO2R to screen out differentially expressed genes (DEGs). H. pylori was detected in 43.3% of the samples. Statistically significant differences were found for IL-6 (P=0.0001) and IL-6R (P=0.0005) genes among the three groups, regardless of the presence of H. pylori. Among patients with H. pylori infection, the IL-6 and IL-6R gene and protein expressions were significantly increased, highlighting IL-6 gene overexpression in patients with GC. No statistically significant differences were found for the rs1800795, rs1800796, and rs1800797 polymorphisms compared to IL-6 gene expression. The results indicated that the IL-6 polymorphisms do not influence its expression, but IL-6 and IL-6R expression seems to be altered by the presence of H. pylori.
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Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Neoplasias Gástricas / Helicobacter pylori / Infecções por Helicobacter / Interleucina-6 / Gastrite Limite: Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Neoplasias Gástricas / Helicobacter pylori / Infecções por Helicobacter / Interleucina-6 / Gastrite Limite: Humans Idioma: En Revista: Braz. j. med. biol. res / Rev. bras. pesqui. méd. biol Assunto da revista: BIOLOGIA / MEDICINA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil