Substitution of Malachite Green with Nigrosin - Eosin Yellow Stain in the Kato-Katz Method: Microscopical Appearance of the Helminth Eggs
Afr. health sci. (Online)
; 7(1): 33-36, 2007.
Article
em En
| AIM
| ID: biblio-1256463
Biblioteca responsável:
CG1.1
ABSTRACT
Background:
The Kato-Katz thick smear technique is the standard technique recommended by the World Health Organisation for the quantitative diagnosis of Schistosoma mansoni and other intestinal helminth infections.The major problem of the technique is that a few hours after the preparation of slides hookworm eggs over clear and disappear due glycerin.Objective:
To illustrate clear visibility of different helminth eggs microscopically in Odongo-Aginya method; substitution of malachite green with 7.5nigrosin in 10formalin and 5eosin in 10formalin.Method:
Measured; strained stool specimen was stained with mixture of nigrosin/ eosin and covered with cellophane cover slips.The prepared slide was examined immediately microscopically.Result:
Slides prepared with Odongo-Aginya method can be examined immediately or later without compromising the visibility of parasite eggs and larvae. Hookworm eggs remain visible for a long time.Conclusion:
The present publication shows microscopic appearance of the helminth eggs using the Odongo-Aginya modification
Texto completo:
1
Base de dados:
AIM
Assunto principal:
Helmintos
Idioma:
En
Revista:
Afr Health Sci
/
Afr. health sci. (Online)
/
African health sciences
/
African health sciences (Online)
/
African health sciences (Print)
Ano de publicação:
2007
Tipo de documento:
Article