Validation of a Rapid Tuberculosis PCR Assay for Detection of MDR-TB Patients in Gauteng; South Africa
S. Afr. j. infect. dis. (Online)
; 25(2): 12-15, 2010.
Artigo
em Inglês
| AIM (África)
| ID: biblio-1270625
Biblioteca responsável:
CG1.1
ABSTRACT
Nucleic acid amplification tests offer shorter turnaround times for diagnosis of tuberculosis (TB) and drug resistance of isolates compared to conventional culture methods. The rapid molecular-based multidrug-resistant (MDR)-TB assay; GenoTyper MTBDRplus (Hain Lifescience) was evaluated in Gauteng; South Africa; as a pilot investigation to assess its performance for detection of MDR-TB in patients who were at high risk of drug-resistant TB. A total of 945 sputum specimens sequentially received within a period of six weeks from seven hospitals were assessed by MTBDRplus and compared to liquid culture drug susceptibility tests (DST) using the MGIT 960 system (BD Diagnostic Systems) as the `gold standard'. Of the 945 specimens processed; 731 had interpretable results from both tests and therefore were included in the analysis. The overall sensitivities of the MTBDRplus in detecting individual resistance to rifampicin (RMP) and isoniazid (INH); as well as MDR were 95.0; 93.4and 100; respectively. The specificities were 99.7for RMP; and 100for INH and MDR. The Genotyper MTBDRplus assay showed excellent concordance with the conventional `gold standard' MGIT DST; and it detected all the MDR-TB cases analysed
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Tuberculose
Base de dados:
AIM (África)
Assunto principal:
Escarro
/
Tuberculose
/
Reação em Cadeia da Polimerase
/
Diagnóstico Precoce
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
S. Afr. j. infect. dis. (Online)
Ano de publicação:
2010
Tipo de documento:
Artigo