High Frequency of Cryptosporidium hominis Infecting Infants Points to A Potential Anthroponotic Transmission in Maputo, Mozambique
PloS pathog
; 20(2): 1-12, Mar 4, 2022. tab, ilus, graf
Artigo
em Inglês
| RDSM
| ID: biblio-1358013
Biblioteca responsável:
MZ1.1
ABSTRACT
Cryptosporidium is one of the most important causes of diarrhea in children less than 2 years of age. In this study, we report the frequency, risk factors and species of Cryptosporidium detected by molecular diagnostic methods in children admitted to two public hospitals in Maputo City, Mozambique. We studied 319 patients under the age of five years who were admitted due to diarrhea between April 2015 and February 2016. Single stool samples were examined for the presence of Cryptosporidium spp. oocysts, microscopically by using a Modified Ziehl-Neelsen (mZN) staining method and by using Polymerase Chain Reaction and Restriction Fragment Length Polymorphism (PCR-RFLP) technique using 18S ribosomal RNA gene as a target. Overall, 57.7% (184/319) were males, the median age (Interquartile range, IQR) was 11.0 (7-15) months. Cryptosporidium spp. oocysts were detected in 11.0% (35/319) by microscopy and in 35.4% (68/192) using PCR-RFLP. The most affected age group were children older than two years, [adjusted odds ratio (aOR) 5.861; 95% confidence interval (CI) 1.532-22.417; p-value < 0.05]. Children with illiterate caregivers had higher risk of infection (aOR 1.688; 95% CI 1.001-2.845; p-value < 0.05). An anthroponotic species C. hominis was found in 93.0% (27/29) of samples. Our findings demonstrated that cryptosporidiosis in children with diarrhea might be caused by anthroponomic transmission.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Moçambique
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Diarreia
/
Doenças Negligenciadas
/
Zoonoses
Base de dados:
RDSM
Assunto principal:
Criptosporidiose
/
Cryptosporidium
/
Diarreia Infantil
Tipo de estudo:
Estudo diagnóstico
/
Estudo de etiologia
/
Fatores de risco
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
PloS pathog
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Instituto de higiene e medicina tropical, universidade nova de lisboa/PT
/
Instituto nacional de saúde/MZ
/
Laboratório interdisciplinar de pesquisas médicas, Instituto oswaldo cruz-FIOCRUZ/BR