Genetic characterisation of south african and mozambican bovine rotaviruses reveals a typical bovine-like artiodactyl constellation derived through multiple reassortment events
PloS pathog
; 20(2): 1-16, Mar 4, 2022. tab, mapas, ilus
Artigo
em Inglês
| RDSM
| ID: biblio-1358116
Biblioteca responsável:
MZ1.1
ABSTRACT
This study presents whole genomes of seven bovine rotavirus strains from South Africa and Mozambique. Double-stranded RNA, extracted from stool samples without prior adaptation to cell culture, was used to synthesise cDNA using a self-annealing anchor primer ligated to dsRNA and random hexamers. The cDNA was subsequently sequenced using an Illumina MiSeq platform without prior genome amplification. All strains exhibited bovine-like artiodactyl genome constellations (G10/G6-P[11]/P[5]-I2-R2-C2-M2-A3/A11/A13-N2-T6-E2-H3). Phylogenetic analysis revealed relatively homogenous strains, which were mostly related to other South African animal strains or to each other. It appears that these study strains represent a specific bo-vine rotavirus population endemic to Southern Africa that was derived through multiple reassortment events. While one Mozambican strain, MPT307, was similar to the South African strains, the second strain, MPT93, was divergent from the other study strains, exhibiting evi-dence of interspecies transmission of the VP1 and NSP2 genes. The data presented in this study not only contribute to the knowledge of circulating African bovine rotavirus strains, but also em-phasise the need for expanded surveillance of animal rotaviruses in African countries in order to improve our understanding of rotavirus strain diversity
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Moçambique
Base de dados:
RDSM
Assunto principal:
Infecções por Rotavirus
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Doenças dos Bovinos
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Genoma Viral
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Rotavirus
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Genótipo
Limite:
Animais
País/Região como assunto:
África
Idioma:
Inglês
Revista:
PloS pathog
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Biochemistry, focus area human metabolomics, north-west university/ZA
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Department of microbiology and biochemistry, university of the free state/MZ
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Department of microbiology and biochemistry, university of the free state/ZA
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Diarrhoeal Pathogens research unit, department of virology, sefako makgatho health sciences university/ZA
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Enteric diseases group, murdoch children's research cnstitute/AU
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Instituto nacional de saúde/MZ
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Next generation sequencing unit, university of the free State/ZA