Sperm DNA damage compromises embryo development, but not oocyte fertilisation in pigs
Biol. Res
; 55: 15-15, 2022. ilus, tab
Artigo
em Inglês
| LILACS
| ID: biblio-1383917
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
The assessment of sperm DNA integrity has been proposed as a complementary test to conventional mammalian semen analysis. In this sense, single-strand (SSB) and double-strand (DSB) DNA breaks, the two types of sperm DNA fragmentation (SDF), have been reported to have different aetiologies and to be associated to different fertility outcomes in bovine and humans. Considering that no studies in porcine have addressed how SDF may affect sperm quality and fertility outcomes, the present work aimed to determine the impact of global DNA damage, SSB and DSB on sperm quality and in vitro fertilising ability. To this end, 24 ejaculates (one per boar) were split into three aliquots the first was used to assess sperm quality parameters through a computer-assisted sperm analysis (CASA) system and flow cytometry; the second was used to perform in vitro fertilisation, and the third, to evaluate sperm DNA integrity using alkaline and neutral Comet assays.RESULTS:
The results showed that global DNA damage negatively correlates (P 0.05).CONCLUSION:
Considering all these findings, this work sets a useful model to study how SDF negatively influences fertility.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Espermatozoides
/
Dano ao DNA
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2022
Tipo de documento:
Artigo
País de afiliação:
Itália
/
Espanha
Instituição/País de afiliação:
Department of Veterinary Medical Sciences/IT
/
University of Girona/ES