Glucose-6-phosphate Metabolic Preferential Destinations in Bovine Oviduct Cells

ABSTRACT

The oviduct is a dynamic organ which facilitates gamete function, fertilization and embryo development. This organ is covered by an epithelium containing ciliated and non-ciliated cells. Secretions of non-ciliated cells compose the oviduct fluid, which will nourish the early embryo. During the period of ovulation, the oviduct exhibits an active role, where the lumen provides an environment suitable for fertilization and the muscle layer contracts rhythmically to move the egg toward the uterus. In this study we aimed to investigate the content of fuel metabolites and enzyme activity assays related to the glycolytic metabolism in bovine oviduct cells such as Glucose-6-phosphate, Glycogen, Pyruvate, Hexokinase, Pyruvate, kinase, Phosphoenolpyruvate carboxykinase and Glucose-6-phosphate dehydrogenase.