Comparison of three protocols to obtain platelet rich plasma (PRP) with rabbits as experimental model / Comparação entre três protocolos de obtenção de plasma rico em plaquetas (PRP) utilizando o coelho como modelo experimental

ABSTRACT

Background: Platelet rich plasma (PRP) is a blood-derived source of growth factors and several cytokines, which are essential for tissue regeneration and important for wound healing due to their angiogenic, mitogenic, and chemotactic activities. To date no protocol has been established for PRP production. Standardization of this technique should consider fundamental factors such as experimental model used, blood collection method, anticoagulant choice, rotation and amount of centrifugations, elapsed time between sample activation and its clinical use in order to ensure quality and biological effects of the product. This study aimed to compare three protocols for PRP achievement in order to evaluate platelet enrichment ability and method reproducibility for further use in clinical investigations regarding PRP therapeutic properties. Materials, Methods & Results: New Zealand higid rabbits whole blood was collected in tubes containing sodium citrate. Samples were obtained through exsanguination, via abdominal aortic puncture, and separated in four aliquots designed for PRP processing and basal platelet count. The count was conducted at the time blood was collected and after every concentration protocol. Methods were tested in triplicates, and three different individuals repeated each technique for three times, reaching 27 repetitions. Selected methodologies consisted in two c

RESUMO

O plasma rico em plaquetas derivado de sangue autólogo é definido como um volume de plasma com uma concentração plaquetária acima dos níveis fisiológicos. É uma fonte autógena e de baixo custo de fatores de crescimento (FC). FC são moléculas bioativas fundamentais no reparo e regeneração de diversos tecidos, capazes de estimular a mitogênese, angiogênese, quimiotaxia, proliferação e diferenciação celular. Entre os fatores liberados pelas plaquetas destacam-se Fator de Crescimento Derivado de Plaquetas (PDGF), Fator de Crescimento Transformador Beta (TGF- ), Fator de Crescimento Endotelial Vascular (VEGF) e Fator de Crescimento Epitelial (EGF). [...]