Natural coinfection of Pseudomonas aeruginosa and Enteroaggregative Escherichia coli in a feral pigeon (Columba livia)
Acta sci. vet. (Impr.)
; 45(suppl.1): 1-4, 2017. ilus
Article
em En
| VETINDEX
| ID: biblio-1457770
Biblioteca responsável:
BR68.1
ABSTRACT
Background:
Rock pigeon (Columba livia) is an exotic and invasive species of the Columbidae family found in several cities around the world, including Brazilian cities, and close to humans. This species is found in large populations often in public locations, where people may have contact. Several studies have demonstrated the isolation of human pathogens from these birds. However, there are scarce studies describing infections by Gram-negative bacteria to which pigeons are susceptible. Therefore, this report aimed to describe a case of natural coinfection of Pseudomonas aeruginosa and Enteroaggregative Escherichia coli in a feral pigeon. Case A sick feral pigeon was delivered at the Laboratory of Ornithological Studies, State University of Ceará, Fortaleza, Brazil. Due to poor prognosis, the individual was euthanized with ketamine via intravenous injection and submitted to necropsy, in which samples were collected for microbiological and histopathological procedure. The procedure was performed aseptically and samples were collected from intestine, liver, spleen, heart and lung. Bacterial isolation was performed with culture media selective for Gram negative bacteria and strains were identified biochemically. Histopathological examination was performed with conventional method and slides were stained with hematoxylin and eosin. DNA from E. coli isolates was extracted with simples boiling method and submitted to uniplex conventional polymerase chain reaction (PCR) to diagnose diarrheagenic pathotypes with specific primers for the following genes aaiC, aatA, eaeA, stx1, stx2, eltB, estA and ipaH. Escherichia coli was isolated from the intestine, liver, spleen and lung, while Pseudomonas aeruginosa was present in liver, spleen and lung. E. coli strains from liver and spleen were positive for the aaiC gene, which is a diagnostic gene for Enteroaggregative Escherichia coli (EAEC) pathotype. [ ]Palavras-chave
Texto completo:
1
Base de dados:
VETINDEX
Assunto principal:
Columbidae
/
Pseudomonas aeruginosa
/
Escherichia coli
/
Coinfecção
Limite:
Animals
Idioma:
En
Revista:
Acta sci. vet. (Impr.)
Ano de publicação:
2017
Tipo de documento:
Article