Hematological and biochemical profile of sheep supplemented with salinomycin submitted to experimental lactic ruminal acidosis / Perfil hematológico e bioquímico de ovinos suplementados com salinomicina submetidos à acidose láctica ruminal

ABSTRACT

Twelve sheep were distributed into two groups, control (CG) and salinomycin (SG), to study clinical, hematological and biochemical alterations in sheep supplemented with the ionophore and to evaluate its effect in preventing experimental lactic ruminal acidosis which was induced with sucrose. Variables were analyzed at intervals of 4h, 8h, 12h, 16h, 24h, 32h and 48h post-induction (PI). The enzymes AST, GGT, ALP and CK were determined, and seric total protein (TP), albumin, urea, creatinine, blood count, total plasmatic protein (TPP), fibrinogen (PF), glucose and L-lactate were quantified. Clinical manifestations of lactic acidosis and lower ruminal pH values were observed 8h PI, with (P 0.05) in SG compared to the basal moment. Neutrophils showed higher scores (P 0.05) in CG compared to SG 4h PI. The PF reached significant values (P 0.05) in CG 48h PI compared to SG. Urea decreased (P 0.05) in both groups 12h PI. Glucose increased (P 0.05) when compared to CG at basal moment. There was a decrease (P 0.05) at urinary pH 12h up to 48h PI, compared to 0h time in CG, while the SG decreased (P 0.05) just at moments 12h and 16h PI. Salinomycin did not prevent acidosis, however, favored the reestablishment of the animals that received it.KEYWORDS clinical biochemistry; fermentation disturbance; ionophores; small ruminants.

RESUMO

Utilizaram-se 12 ovinos distribuídos nos grupos controle (GC) e no salinomicina (GS) com objetivo de estudar alterações clínicas, hematológicas e bioquímicas nos ovinos suplementados com o ionóforo e avaliar seu efeito na prevenção da acidose ruminal experimental. Induziu-se acidose ruminal com sacarose e as variáveis foram analisadas 4h, 8h, 12h, 16h, 24h, 32h e 48h pós-indução (PI). Determinaram-se as enzimas AST, GGT, FA, CK, as proteínas totais séricas (PT), albumina, ureia, creatinina, hemograma, proteína plasmática total (PPT), fibrinogênio (FP), glicose e L-lactato. Manifestações clínicas de acidose láctica ruminal e os menores valores de pH foram observadas 8h PI, com (P 0,05) no GS comparado ao momento 0h. Os neutrófilos apresentaram maiores contagens (P 0,05) no GC 4h PI comparado ao GS. O FP alcançou maiores valores (P 0,05) no GC 48h PI comparado ao GS. A uréia diminuiu (P 0,05) em ambos os grupos 12h PI. A glicose aumentou (P 0,05) no GC comparado ao momento 0h. Houve queda (P 0,05) do pH urinário no momento 12h até 48h PI, em relação ao momento 0h no GC, enquanto no GS apenas os momentos 12h e 16h PI apresentaram diminuição (P 0,05). A salinomicina não preveniu a acidose; no entanto, favoreceu o restabelecimento dos animais tratados.PALAVRAS-CHAVE Bioquímica clínica; distúrbio fermentativo; ionóforos; pequenos ruminantes.