Light-induced injury in mouse embryos revealed by single-cell RNA sequencing
Biol. Res
; 52: 48-48, 2019. ilus, graf
Artigo
em Inglês
| LILACS
| ID: biblio-1505768
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Light exposure is a common stress factor in in vitro manipulation of embryos in the reproductive center. Many studies have shown the deleterious effects of high-intensity light exposure in different animal embryos. However, no transcriptomic studies have explored the light-induced injury and response in preimplantation embryos.RESULTS:
Here, we adopt different time-courses and illumination intensities to treat mouse embryos at the 2-cell stage and evaluate their effects on blastulation. Meanwhile, single-cell transcriptomes from the 2-cell to blastocyst stage were analyzed after high-intensity light exposure. These data show that cells at each embryonic stage can be categorized into different light conditions. Further analyses of differentially expressed genes and GO terms revealed the light-induced injury as well as the potential repair response after high-intensity lighting. Maternal-to-zygote transition is also affected by the failure to remove maternal RNAs and deactivate zygotic genome expression.CONCLUSION:
Our work revealed an integrated response to high-intensity lighting, involving morphological changes, long-lasting injury effects, and intracellular damage repair mechanisms.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Análise de Sequência de RNA
/
Técnicas de Cultura Embrionária
/
Desenvolvimento Embrionário
/
Análise de Célula Única
/
Luz
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
China
/
Holanda
Instituição/País de afiliação:
Avans University of Applied Sciences/NL
/
Tongji University/CN