Degradation of mitochondrial structure and deficiency of complex I were associated with the transgenic CMS of rice
Biol. Res
; 54: 6-6, 2021. ilus, graf, tab
Article
em En
| LILACS
| ID: biblio-1505798
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Mitochondria play a significant role in plant cytoplasmic male sterility (CMS). In our previous study, mitochondrial complex I genes, nad4, nad5, and nad7 showed polymorphisms between the transgenic CMS line M2BS and its wild type M2B. The sterility mechanism of the M2BS at cytological, physiological, biochemical, and molecular level is not clear.RESULTS:
Cytological observation showed that the anthers were light yellow, fissured, invalid in KI-I2, and full of irregularly typical abortion pollen grains in M2BS. Transmission electron microscopic (TEM) observation revealed no nucleus and degraded mitochondria with obscure cristae in anther cells of M2BS. The results of staining for H2O2 presented a large number of electron dense precipitates (edp) in intercellular space of anther cells of M2BS at anthesis. Moreover, the anther respiration rate and complex I activity of M2BS were significantly lower than those of wild type M2B during pollen development. Furthermore, RNA editing results showed only nad7 presented partially edited at 534th nucleotides. The expression of nad5 and nad7 revealed significant differences between M2B and M2BS.CONCLUSIONS:
Our data demonstrated that mitochondrial structural degradation and complex I deficiency might be associated with transgenic CMS of rice.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Oryza
/
Complexo I de Transporte de Elétrons
/
Infertilidade das Plantas
/
Mitocôndrias
Tipo de estudo:
Risk_factors_studies
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Chile