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Mvda is required for zebrafish early development
Wong, Wenghong; Huang, Yike; Wu, Zhuanbin; Kong, Yu; Luan, Jing; Zhang, Qiaoan; Pan, Jiewen; Yan, Kexiang; Zhang, Zhenghua.
Afiliação
  • Wong, Wenghong; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Huang, Yike; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Wu, Zhuanbin; Shanghai Model Organisms Center Inc. Shanghai. CN
  • Kong, Yu; Chinese Academy of Science. Institute of Neuroscience. Shanghai. CN
  • Luan, Jing; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Zhang, Qiaoan; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Pan, Jiewen; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Yan, Kexiang; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
  • Zhang, Zhenghua; Shanghai Medical College of Fudan University. Huashan Hospital. Department of Dermatology. Shanghai. CN
Biol. Res ; 54: 17-17, 2021. ilus
Artigo em Inglês | LILACS | ID: biblio-1505810
Biblioteca responsável: CL1.1
ABSTRACT

BACKGROUND:

The MVD gene mutations are identified in porokeratosis, which is considered a skin-specific autoin- flammatory keratinization disease. However, the biological function of MVD gene remains largely unknown. Therefore, we analyzed the function of mvda gene, orthologous to the human MVD gene, in developing zebrafish.

METHODS:

Morpholino antisense oligonucleotide technique was used to generate mvda loss-of-function phenotypes. Knockdown of mvda was confirmed by RT-PCR and Sanger sequencing. Scanning and transmission electron microscopy were performed to analyze the morphology of the epidermis. Angiogenesis study was presented using the Tg(fli1aEGFP)yl transgenic strain. In addition, acridine orange staining was used to examine the apoptotic cells in vivo.

RESULTS:

As expected, the mvda morphants showed abnormal morphology of the epidermis. Moreover, we observed ectopic sprouts in trunk angiogenesis and impaired formation of the caudal vein plexus in the mvda-deficient zebrafish. Besides, increased apoptosis was found throughout the tail, heart, and eyes in mvda zebrafish morphants.

CONCLUSIONS:

These findings indicated the essential role of mvda in the early development of zebrafish. This was the first in vivo knockdown study of the zebrafish mvda gene, which might offer insight into the biological function of the human MVD gene.
Assuntos


Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Peixe-Zebra Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Chinese Academy of Science/CN / Shanghai Medical College of Fudan University/CN / Shanghai Model Organisms Center Inc/CN

Texto completo: Disponível Coleções: Bases de dados internacionais Base de dados: LILACS Assunto principal: Peixe-Zebra Tipo de estudo: Estudo prognóstico Limite: Animais / Humanos Idioma: Inglês Revista: Biol. Res Assunto da revista: Biologia Ano de publicação: 2021 Tipo de documento: Artigo País de afiliação: China Instituição/País de afiliação: Chinese Academy of Science/CN / Shanghai Medical College of Fudan University/CN / Shanghai Model Organisms Center Inc/CN
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