Fasudil mediates neuroprotection in ischemia/reperfusion by modulating the ROCK-PPARα-NOX axis
Acta cir. bras
; 38: e387023, 2023. tab, graf, ilus
Artigo
em Inglês
| LILACS, VETINDEX
| ID: biblio-1527595
Biblioteca responsável:
BR68.1
Localização: BR68.1
ABSTRACT
Purpose:
Cerebral ischemia-reperfusion (I/R) is a neurovascular disorder that leads to brain injury. In mice, Fasudil improves nerve injury induced by I/R. However, it is unclear if this is mediated by increased peroxisome proliferator-activated receptor-α (PPARα) expression and reduced oxidative damage. This study aimed to investigate the neuroprotective mechanism of action of Fasudil.Methods:
MCAO (Middle cerebral artery occlusion) was performed in male C57BL/6J wild-type and PPARα KO mice between September 2021 to April 2023. Mice were treated with Fasudil and saline; 2,3,5-Triphenyltetrazolium chloride (TTC) staining was performed to analyze cerebral infarction. PPARα and Rho-associated protein kinase (ROCK) expression were detected using Western blot, and the expression of NADPH subunit Nox2 mRNA was detected using real-time polymerase chain reaction. The NADPH oxidase activity level and reactive oxygen species (ROS) content were also investigated.Results:
After cerebral ischemia, the volume of cerebral necrosis was reduced in wild-type mice treated with Fasudil. The expression of PPARα was increased, while ROCK was decreased. Nox2 mRNA expression, NADPH oxidase activity, and ROS content decreased. There were no significant changes in cerebral necrosis volumes, NADPH oxidase activity, and ROS content in the PPARα KO mice treated with Fasudil.Conclusions:
In mice, the neuroprotective effect of Fasudil depends on the expression of PPARα induced by ROCK-PPARα-NOX axis-mediated reduction in ROS and associated oxidative damage.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
/
VETINDEX
Assunto principal:
Lesões Encefálicas
/
Traumatismo por Reperfusão
/
Isquemia Encefálica
/
Estresse Oxidativo
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2023
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
The First Affiliated hospital of Dali University/CN