Validation of QF-PCR for prenatal diagnoses in a Brazilian population
Clinics
; 72(7): 400-404, July 2017. tab
Artigo
em Inglês
| LILACS
| ID: biblio-890711
Biblioteca responsável:
BR1.1
ABSTRACT
OBJECTIVES:
Quantitative fluorescence polymerase chain reaction (QF-PCR) is a rapid and reliable method for screening aneuploidies, but in Brazil, it is not used in public services. We investigated the accuracy of QF-PCR for the prenatal recognition of common aneuploidies and compared these results with cytogenetic results in our laboratory.METHOD:
A ChromoQuant QF-PCR kit containing 24 primer pairs targeting loci on chromosomes 21, 13, 18, X and Y was employed to identify aneuploidies of the referred chromosomes.RESULTS:
A total of 162 amniotic fluid samples analyzed using multiplex QF-PCR were compared with karyotyping analysis. The QF-PCR results were consistent with the results of cytogenetic analysis in 95.4% of all samples.CONCLUSION:
QF-PCR was demonstrated to be efficient and reliable for prenatal aneuploidy screening. This study suggests that QF-PCR can be used as a rapid diagnostic method. However, rearrangements and some mosaic samples cannot be detected with this test; thus, those exceptions must undergo cytogenetic analysis.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Diagnóstico Pré-Natal
/
Reação em Cadeia da Polimerase
/
Aneuploidia
Tipo de estudo:
Estudo diagnóstico
/
Estudo observacional
Limite:
Adolescente
/
Adulto
/
Feminino
/
Humanos
/
Gravidez
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Clinics
Assunto da revista:
Medicina
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR