Your browser doesn't support javascript.
loading
Granulocyte colony-stimulating factor combined with Methylprednisolone improves functional outcomes in rats with experimental acute spinal cord injury
Teixeira, William Gemio Jacobsen; Cristante, Alexandre Fogaça; Marcon, Raphael Martus; Bispo, Gustavo; Ferreira, Ricardo; de Barros-Filho, Tarcísio Eloy Pessoa.
Afiliação
  • Teixeira, William Gemio Jacobsen; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
  • Cristante, Alexandre Fogaça; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
  • Marcon, Raphael Martus; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
  • Bispo, Gustavo; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
  • Ferreira, Ricardo; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
  • de Barros-Filho, Tarcísio Eloy Pessoa; Universidade de São Paulo. Hospital das Clinicas. Divisao de Cirurgia da Coluna. Sao Paulo. BR
Clinics ; Clinics;73: e235, 2018. tab, graf
Article em En | LILACS | ID: biblio-890742
Biblioteca responsável: BR1.1
ABSTRACT

OBJECTIVES:

To evaluate the effects of combined treatment with granulocyte colony-stimulating factor (G-CSF) and methylprednisolone in rats subjected to experimental spinal cord injury.

METHODS:

Forty Wistar rats received a moderate spinal cord injury and were divided into four groups control (no treatment); G-CSF (G-CSF at the time of injury and daily over the next five days); methylprednisolone (methylprednisolone for 24 h); and G-CSF/Methylprednisolone (methylprednisolone for 24 h and G-CSF at the time of injury and daily over the next five days). Functional evaluation was performed using the Basso, Beattie and Bresnahan score on days 2, 7, 14, 21, 28, 35 and 42 following injury. Motor-evoked potentials were evaluated. Histological examination of the spinal cord lesion was performed immediately after euthanasia on day 42.

RESULTS:

Eight animals were excluded (2 from each group) due to infection, a normal Basso, Beattie and Bresnahan score at their first evaluation, or autophagy, and 32 were evaluated. The combination of methylprednisolone and G-CSF promoted greater functional improvement than methylprednisolone or G-CSF alone (p<0.001). This combination also exhibited a synergistic effect, with improvements in hyperemia and cellular infiltration at the injury site (p<0.001). The groups displayed no neurophysiological differences (latency p=0.85; amplitude p=0.75).

CONCLUSION:

Methylprednisolone plus G-CSF promotes functional and histological improvements superior to those achieved by either of these drugs alone when treating spinal cord contusion injuries in rats. Combining the two drugs did have a synergistic effect.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Traumatismos da Medula Espinal / Metilprednisolona / Fator Estimulador de Colônias de Granulócitos / Fármacos Neuroprotetores Tipo de estudo: Evaluation_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Clinics Assunto da revista: MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil

Texto completo: 1 Coleções: 01-internacional Base de dados: LILACS Assunto principal: Traumatismos da Medula Espinal / Metilprednisolona / Fator Estimulador de Colônias de Granulócitos / Fármacos Neuroprotetores Tipo de estudo: Evaluation_studies / Prognostic_studies Limite: Animals Idioma: En Revista: Clinics Assunto da revista: MEDICINA Ano de publicação: 2018 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil