Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
Rev. Soc. Bras. Med. Trop
; 51(2): 168-173, Mar.-Apr. 2018. graf
Artigo
em Inglês
| LILACS
| ID: biblio-897058
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year.METHODS:
In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR).RESULTS:
All three assays identified 29.1% (39/134) of the patients as dengue-positive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods.CONCLUSIONS:
The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Dengue
/
Doenças Negligenciadas
Base de dados:
LILACS
Assunto principal:
Leucócitos Mononucleares
/
Dengue
/
Vírus da Dengue
/
Anticorpos Antivirais
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
Medicina Tropical
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade de São Paulo/BR