Profile of exoantigens from clinical isolates of Paracoccidioides lutzii IN Mato Grosso, Brazil
Rev. patol. trop
; 45(3): 265-272, set. 2016. tab, ilus
Artigo
em Inglês
| LILACS
| ID: biblio-913231
Biblioteca responsável:
BR816.9
ABSTRACT
The objective of this study was to determine the immunochemical profile of three exoantigen preparations obtained from clinical samples of Paracoccidioides lutzii from the state of Mato Grosso, Brazil. The exoantigens were prepared according to a protocol standardized by Camargo et al. and their immunochemical profiles were analyzed using SDS-PAGE. The observed protein bands exhibited molecular weights of 20, 30, and 130 kDa. The 130 kDa glycoprotein was detected in all three preparations. The detection of this high-molecular-weight protein suggests a distinct immunochemical profile. These differences may help to explain the false negative reactions obtained when using the antigen from P. brasiliensis reference strain B-339. This information is essential for increasing the specificity of serological tests used to monitor the prognosis of patients with paracoccidioidomycosis.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Paracoccidioides
Tipo de estudo:
Guia de prática clínica
País/Região como assunto:
América do Sul
/
Brasil
Idioma:
Inglês
Revista:
Rev. patol. trop
Assunto da revista:
Medicina Tropical
/
Patologia
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Assistant Secretary of Policy and Health Care Department of Health, State Department of Health, SES/BR
/
Department of Epidemiological Surveillance Coordination, Central Laboratory (LACEN-MT)/BR
/
Mycology Laboratory, Federal University of São Paulo (UNIFESP)/BR
/
Post-Graduate Program in Health Sciences, Faculty of Medicine, Federal University of Mato Grosso/BR