Proliferation of human adipose tissue-derived stem cells stimulated by oil rich in thymol of Lippia origanoides
Acta cir. bras
; 33(5): 431-438, May 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-949342
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate the effects of this thymol-rich oil in the proliferation of human adipose tissue-derived stem cells.Methods:
Stem cells were isolated from human adipose tissue by liposuction. After the first passage, cells were cultivated in triplicate for three days in control medium and medium supplemented with three oil samples (1.0 μg/mL, 5.0 μg/mL, and 25.0 μg/mL). Cells were analyzed by the MTT assay at passage 1 (P1), and cell proliferation of control and 1 μg/mL groups was determined with a hemocytometer at P2 and P3.Results:
Viability of the essential oil-treated cells was significantly higher than the control group at P1 (p = 0.0008). The treatment with the oil, at a concentration of 1 µg/mL, led to increases of 24.8% at P1 and 43.0% at P3 in the rate of cell proliferation compared with control cells.Conclusion:
Supplementing culture medium with essential oil of Lippia origanoides increased cell proliferation, especially at later passages.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Timol
/
Óleos de Plantas
/
Óleos Voláteis
/
Lippia
/
Proliferação de Células
/
Antioxidantes
Limite:
Adulto
/
Humanos
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UFPA/BR
/
Universidade Federal University do Pará/BR
/
Universidade Federal de São Paulo/BR
/
Universidade Federal do Oeste do Pará/BR