Simultaneous application of BrdU and WST-1 measurements for detection of the proliferation and viability of airway smooth muscle cells
Biol. Res
; 47: 1-5, 2014. ilus, graf
Artigo
em Inglês
| LILACS
| ID: biblio-950771
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
BrdU is a commonly used reagent in cell proliferation assays, and WST-1 measurement is widely used to detect cell viability. However, no previous study has formally reported the combination of the two assays, which may be used to detect the proliferation and viability simultaneously. In this study, we examined the effect of adding BrdU 2 h prior to the WST-1 assay and tried to test the possibility of the combined detection using rat airway smooth muscle cells.RESULTS:
The WST-1 measurements obtained from the combined detection were consistent with those obtained from the separate detection, which suggested that the addition of BrdU 2 h prior to the WST-1 analysis did not affect the WST-1 results. The BrdU measurements obtained from the combined detection also demonstrated the same trend as that obtained from the separate detection, and dosages of 200, 400 and 800 ng/ml testing reagent significantly inhibited the proliferation of rat airway smooth muscle cells.CONCLUSIONS:
Our study suggests that the BrdU and WST-1 measurements can be applied simultaneously without mutual interference, which may increase the efficacy and consistency of these measurements to a certain extent.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Avaliação da Tecnologia Biomédica
/
Sais de Tetrazólio
/
Traqueia
/
Bromodesoxiuridina
/
Miócitos de Músculo Liso
/
Proliferação de Células
Tipo de estudo:
Estudo diagnóstico
/
Avaliação de tecnologias de saúde
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Shanghai University of Traditional Chinese Medicine/CN