Influence of low-level laser irradiation on osteocalcin protein and gene expression in bone tissue1
Acta cir. bras
; 33(9): 736-743, Sept. 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-973502
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract Purpose:
To evaluate osteocalcin gene and protein expression in vitro and in an in vivo model of ostectomy.Methods:
Twenty Wistar rats were assigned into two groups A (n=10, laser) and B (n=10, control). Ostectomy was performed in the femur diaphysis; the twenty fragments removed, composed in vitro groups named as in vivo (A and B) and cultivated in CO2 atmosphere for thirteen days. Low-level laser irradiation was performed in groups A (in vivo and in vitro) by an GaAlAs device (λ=808 nm, dose of 2J/cm2, power of 200mW, power density of 0.2W/cm2, total energy of 1.25J, spot diameter of 0.02mm) for 5 seconds, at one point, daily. It was performed immunocytochemistry assays in vivo and in vitro groups. In vitro groups were also submitted to RNA extraction, cDNA synthesis and gene expression by quantitative PCR. Statistical analysis was realized with p<0.05.Results:
Immunocytochemistry scores showed no significant differences between control and laser groups either in vivo and in vitro. Gene expression also showed no statistical differences.Conclusion:
Low-level laser irradiation did not alter osteocalcin protein and gene expression in vivo and in vitro in the studied period but it may have been expressed them in an earlier period.
Texto completo:
Disponível
Coleções:
Bases de dados internacionais
Base de dados:
LILACS
Assunto principal:
Expressão Gênica
/
Osteocalcina
/
Fêmur
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
UNIFESP/BR
/
Universidade Federal de São Paulo/BR
/
Universidade Federal dos Vales do Jequitinhonha e Mucuri/BR