Bloqueo del receptor del factor de crecimiento semejante a la insulina tipo I utilizando oligodeoxinucleótidos antisentido en cáncer de mama experimental / Type I insulin-like growth factor receptor antisense strategies in experimental breast cancer
Medicina (B.Aires)
; 64(2): 129-134, 2004. graf, ilus
Artigo
em Espanhol
| BINACIS
| ID: bin-123261
Biblioteca responsável:
BR1.1
ABSTRACT
We addressed the effect of targeting type I insulin-like growth factor receptor (IGF-IR), with antisense strategies in in vivo growth of breast cancer cells. We used C4HD tumors from an experimental model of hormonal carcinogenesis in which medroxyprogesterone acetate induced mammary adenocarcinomas in Balb/c mice. Intratumor or systemic administration of phosphorothiolated antisense oligodeoxynucleotides (AS[S]ODN) to IGF-IR mRNA resulted in a significant inhibition of C4HD tumor growth. The antitumor effect was specific since inhibition of tumor growth was dose-dependent and no effect was observed in mice treated with sense S[S]ODN. Tumors from AS[S]ODN-treated mice showed a decrease in IGF-IR expression and in insulin receptor substrate-1 tyrosine phosphorylation. Activation of PI-3K/Akt, p42/p44 MAPK and ErbB-2 was abolished in tumors treated with AS[S]ODN. Progesterone receptor expression or activity remained invariable. This is the first demonstration that breast cancer growth can be inhibited by direct in vivo administration of IGF-IR AS[S]ODN.(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Argentina
Base de dados:
BINACIS
Assunto principal:
Adenocarcinoma
/
Receptores de Somatomedina
/
Receptor IGF Tipo 1
/
Oligodesoxirribonucleotídeos Antissenso
/
Neoplasias Mamárias Experimentais
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Espanhol
Revista:
Medicina (B.Aires)
Ano de publicação:
2004
Tipo de documento:
Artigo
Instituição/País de afiliação:
CONICET/Argentina