Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers. / Optimization of laccase production by two strains of Ganoderma lucidum using phenolic and metallic inducers.
Rev. Argent. Microbiol.
; 46(2): 144-9, 2014 Apr-Jun.
Artigo
em Espanhol
| BINACIS
| ID: bin-133669
ABSTRACT
Ganoderma lucidum (Curtis) P. Karst is a white rot fungus that is able to degrade the lignin component in wood. The ability of two strains of this species to produce the ligninolytic enzyme laccase was assessed. After the evaluation of induction with heavy metals and phenolic compounds, it was found that among the tested substances, copper and ferulic acid are the best laccase inducers. It was also observed that the two types of inducers (phenolic and metallic) produce different electrophoretic patterns of laccase activity. Optimized concentrations of inducers were obtained through a factorial design and the thermal stability of optimized supernatants was studied at a wide range of acidic pH. We found that the enzyme is more thermostable at higher pH values.
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Argentina
Base de dados:
BINACIS
Assunto principal:
Reishi
/
Lacase
Idioma:
Espanhol
Revista:
Rev. Argent. Microbiol.
Ano de publicação:
2014
Tipo de documento:
Artigo