Producción de glucosa-isomerasa por Streptomyces phaechromogenes. / [Production of glucose isomerase by Streptomyces phaeochromogenes]
Rev. argent. microbiol
; 15(1): 1-8, 1983.
Artigo
em Espanhol
| BINACIS
| ID: bin-49732
Biblioteca responsável:
AR32.1
ABSTRACT
The production of glucose isomerase was studied in the Streptomyces phaeochromogenes NRRL B-3559 strain. The influence of the medium composition and the aeration conditions was determined Selection of colonies was necessary to obtain the best results. A grey colony proved to be the most productive one. It was necessary to use the frozen inocula in order to obtain steady reproduction values. It was possible to achieve the maximum enzymatic level (1600 UE/1 min) using a CoCl2. 6H2O concentration of 0.18 g/l. The aeration studies conducted in a mechanically stirred fermentor showed results comparable to those obtained in shaker flasks, operating at 550 rpm and using an air flow of 1 1/1 min. The culture proved to properly aerated since both the cellular oxygen uptake and oxygen demand were similar (Table 1).
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Base de dados:
BINACIS
Idioma:
Espanhol
Revista:
Rev. argent. microbiol
Ano de publicação:
1983
Tipo de documento:
Artigo