On changes in pancreas cytomorphometry, serum insulin and metabolic adjustments induced by long term orchidectomy and substitutive therapy with testosterone in dogs.

ABSTRACT

Mongrel, male, fasted, unanesthetized dogs under the following alternative treatments 1) nil, 2) orchidectomy 4 months before the study, 3) orchidectomy 10 months in advance, 4) orchidectomy like in (3) followed by i.m. propylenglycol treatment, 0.05 ml/kg body wt./day, 15 days (vehicle controls, and 5) testosterone hemisuccinate in propylenglycol, 0.75 mg in 0.05 ml/kg body wt./day, for 15 days, were used in this study. Pancreas slices of animals of every group were stained with immunoperoxidase. The animals were fasted, anesthetized for pancreas removal. Pancreatic section mean absorbance was estimated in a Zeiss cytospectrophotometer with a coupled computer. Blood sugar (BS) and both serum, immunoreactive insulin (IRI) and free fatty acids (FFA) were assayed in these.