Transgenic wheat plants resistant to herbicide BASTA obtained by microprojectile bombardment
Biocell
; 26(2): 217-223, Aug. 2002.
Article
em En
| BINACIS
| ID: bin-6467
Biblioteca responsável:
BR1.1
ABSTRACT
Wheat (Triticum aestivum) transgenic plants of an important commercial cv (Oasis) was obtained with an efficient and short procedure. First, the optimum conditions for the embryogenic calli generation from immature embryos with a high regeneration percentage were established. The transformation of calli was performed by high velocity microprojectile bombardment, using the pAHC25 plasmid, which contains the reporter gene beta-glucuronidase (GUS) and the selectable BAR gene which confers resistance to the herbicide Basta. The transformations were confirmed by beta-glucuronidase assay activity, PCR and Southern blot analysis. The efficiency of this procedure was high and similar to other reports in which "model" cultivar was used.(AU)
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Coleções:
06-national
/
AR
Base de dados:
BINACIS
Assunto principal:
Triticum
/
Plantas Geneticamente Modificadas
/
Aminobutiratos
/
Herbicidas
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Biocell
Ano de publicação:
2002
Tipo de documento:
Article