Evaluación del efecto inmunomodulador de Bougainvillea xbuttiana (var. naranja) sobre la producción de citoquinas de macrófagos inducida pelo veneno de Bothrops jararaca
Bol Latinoam Caribe Plantas Med Aromat, v. 23, n. 2, p. 199-213, mar. 2024.
Artigo
em Inglês
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: bud-5232
Biblioteca responsável:
BR78.1
ABSTRACT
To study the effect of 50% ethanol extract of Bougainvillea xbuttianaon the enzymatic activity, cell viability and cytokine production provoked by the venom of Bothrops jararacain macro-phages. Three assays were used to study the effects of B. xbuttianaextract on the damage pro-duced by B. jararaca Enzymatic activity was detected by measuring the proteolytic and phos-pholipase A2; macrophages cytotoxicity was determined by the MTT method; levels of cytokine were evaluated using ELISA and a biological assay. After treatment with 300 μg/mL B. xbuttianaextract for 30 min, the proteolytic and phospholipase A2activities of the venom were reduced to 95 and 61%, respectively. In macrophages cultures treated with B. xbuttianaextract combined with venom, the production of TNF-α, IL-6 and IFN-γ was reduced, whereas IL-10 was potenti-ated. Our results support the potential effect of the B. xbuttianaextract as a complementary therapy against the toxicity caused by the venom of B. jararacasnakes.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Base de dados:
Sec. Est. Saúde SP
/
SESSP-IBPROD
Idioma:
Inglês
Revista:
Bol Latinoam Caribe Plantas Med Aromat
Ano de publicação:
2024
Tipo de documento:
Artigo