Characterization of alpha-L-fucosidase and other digestive hydrolases from Biomphalaria glabrata
Acta Trop
; 141: 118-127, 2015.
Artigo
em Inglês
| Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP
| ID: but-ib10043
Biblioteca responsável:
BR78.1
Localização: BR78.1
ABSTRACT
Schistosoma mansoni is one of the major agents of the disease Schistosomiasis, which is one of the major global public health concerns. Biomphalaria glabrata is an obligate intermediate mollusc host of S. mansoni. Although the development of S. mansoni occurs in the snail hepatopancreas, studies that focus on this organ remain limited. In this study, we biochemically identified five distinct carbohydrases (amylase, maltase, alpha-glucosidase, trehalase, and alpha-L-fucosidase), lipases, and peptidases in the B. glabrata hepatopancreas and focused on the isolation and characterization of the activity of alpha-L-fucosidase. The isolated alpha-L-fucosidase has a molecular mass of 141 kDa, an optimum pH of 5.8, and is inhibited by Tris, fucose, and 1-deoxyfuconojirimycin. B. glabrata alpha-L-fucosidase is an exoglycosidase that can hydrolyze the natural substrate fucoidan to fucose residues. It presented K-m values of 48.4 mu M to 4-Methylumbelliferyl alpha-L-fucopyranoside and 0.55 mM to p-nitrophenyl-alpha-L-fucopyranoside. Thus, alpha-L-fucosidase has a high activity in the hepatopancreas of B. glabrata, and the differential expression of this enzyme between susceptible and resistant strains indicates that besides its digestive role, alpha-L-fucosidase may also be important in host/parasite interactions.
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Brasil
Base de dados:
Sec. Est. Saúde SP
/
SESSP-IBPROD
Assunto principal:
Zoologia
/
Bioquímica
/
Biodiversidade
Idioma:
Inglês
Revista:
Acta Trop
Ano de publicação:
2015
Tipo de documento:
Artigo