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Antitumoral effects of Amblyomma sculptum Berlese saliva in neuroblastoma cell lines involve cytoskeletal deconstruction and cell cycle arrest
Nascimento, Thatyanne Gradowski do; Vieira, Priscilla Santos; Cogo, Sheron Campos; Dias-Netipanyj, Marcela Ferreira; França Junior, Nilton de; Câmara, Diana Aparecida Dias; Porcacchia, Allan Saj; Mendonça, Ronaldo Zucatelli; Moreno-Amaral, Andréa Novais; Sá Júnior, Paulo Luiz de; Simons, Simone Michaela; Zischler, Luciana; Elifio-Esposito, Selene.
Afiliação
  • Vieira, Priscilla Santos; Instituto Butantan. Laboratório de Genética.
  • Cogo, Sheron Campos; Instituto Butantan. Laboratório de Genética.
  • Dias-Netipanyj, Marcela Ferreira; Instituto Butantan. Laboratório de Parasitologia.
  • França Junior, Nilton de; Instituto Butantan. Laboratório de Genética.
  • Câmara, Diana Aparecida Dias; Instituto Butantan. Laboratório de Parasitologia.
Rev Bras Parasitol Vet ; 28(1): p. 126-133, 2019.
Artigo em Inglês | Sec. Est. Saúde SP, SESSP-IBPROD, Sec. Est. Saúde SP | ID: but-ib15915
Biblioteca responsável: BR78.1
Localização: BR78.1
ABSTRACT
The antitumor properties of ticks salivary gland extracts or recombinant proteins have been reported recently, but little is known about the antitumor properties of the secreted components of saliva. The goal of this study was to investigate the in vitro effect of the saliva of the hard tick Amblyomma sculptum on neuroblastoma cell lines. SK-N-SK, SH-SY5Y, Be(2)-M17, IMR-32, and CHLA-20 cells were susceptible to saliva, with 80% reduction in their viability compared to untreated controls, as demonstrated by the methylene blue assay. Further investigation using CHLA-20 revealed apoptosis, with approximately 30% of annexin-V positive cells, and G0/G1-phase accumulation (>60%) after treatment with saliva. Mitochondrial membrane potential (??m) was slightly, but significantly (p < 0.05), reduced and the actin cytoskeleton was disarranged, as indicated by fluorescent microscopy. The viability of human fibroblast (HFF-1 cells) used as a non-tumoral control decreased by approximately 40%. However, no alterations in cell cycle progression, morphology, and ??m were observed in these cells. The present work provides new perspectives for the characterization of the molecules present in saliva and their antitumor properties.
Texto completo: Disponível Coleções: Bases de dados nacionais / Brasil Base de dados: Sec. Est. Saúde SP / SESSP-IBPROD Idioma: Inglês Revista: Rev Bras Parasitol Vet Ano de publicação: 2019 Tipo de documento: Artigo
Texto completo: Disponível Coleções: Bases de dados nacionais / Brasil Base de dados: Sec. Est. Saúde SP / SESSP-IBPROD Idioma: Inglês Revista: Rev Bras Parasitol Vet Ano de publicação: 2019 Tipo de documento: Artigo
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