TNF-alpha and IL-10 downregulation and marked oxidative stress in Neuromyelitis Optica
J Inflamm (Lond)
; 6(18)June 2, 2009. graf
Artigo
em Inglês
| CUMED
| ID: cum-39784
Biblioteca responsável:
CU1.1
ABSTRACT
ABSTRACT BACKGROUND:
Neuromyelitis optica is a central nervous system demyelinating and inflammatory syndrome. The objective of this study is to identify cytokines related to the cellular immune response as well as blood brain barrier integrity and oxidative stress.METHODS:
We performed a molecular characterization of cellular immune response and oxidative stress in serum from relapsing-NMO (R-NMO) patients and established the correlations between the clinical measurements and molecular parameters using the Bayesian approach.Serum samples from 11 patients with R-NMO diagnosed according to Wingerchuk criteria and matched in terms of age, gender and ethnicity with the healthy controls were analyzed. The levels of TNF-alpha, IFN-gamma, IL-10, MMP-9, TIMP-1 and oxidative stress markers malondialdehyde, advanced oxidation protein products, peroxidation potential, superoxide dismutase, catalase, and total hydroperoxides were measured.RESULTS:
We found almost undetectable levels of TNF-alpha, a decreased production of IL-10 and a significant up-regulation of every oxidative stress biomarker studied. The insufficient production of TNF-alpha and IL-10 in R-NMO patients, which are two important players of T cell mediated immunoregulation, suggest an effector - regulator imbalance. The overproduction of oxygen reactive species as a consequence of the chronic inflammatory milieu is reflected on the excess of oxidative damage mediators detected. Furthermore, Multidimensional Scaling and a Bayesian linear regression model revealed a significant linear dependence between Expanded Disability Status Scale Kurtzke and TIMP-1; pointing to a possible predictive or prognostic value of this clinical-molecular relationship.CONCLUSION:
These results suggest that there is a breakdown in immunoregulatory mechanisms and noteworthy pro-oxidant environment contributing to NMO pathogenesis(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Cuba
Base de dados:
CUMED
Assunto principal:
Neuromielite Óptica
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Fator de Necrose Tumoral alfa
/
Estresse Oxidativo
Tipo de estudo:
Estudo prognóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
J Inflamm (Lond)
Ano de publicação:
2009
Tipo de documento:
Artigo
Instituição/País de afiliação:
Center for Research and Biological Evaluations/Cuba
/
Clinical Trials Division/Cuba
/
Higher Institute of Medical Sciences Victoria de Girón/Cuba
/
International Center of Neurological Restoration/Cuba