Evaluation of phage assay for rapid phenotypic detection of rifampicin resistance in Mycobacterium tuberculosis / s. t
Ann Clin Microbiol Antimicrob
; 21Abr. 2006. tab, graf
Artigo
em Inglês
| CUMED
| ID: cum-40049
Biblioteca responsável:
CU1.1
ABSTRACT
Conventional methods for susceptibility testing require several months before results can be reported. However, rapid methods to determine drug susceptibility have been developed recently. Phage assay have been reported as a rapid useful tools for antimicrobial susceptibility testing. The aim of this study was to apply the Phage assay for rapid detection of resistance on Mycobacterium tuberculosis strains in Cuba. Phage D29 assay was performed on 102 M. tuberculosis strains to detect rifampicin resistance. The results were compared with the proportion method (gold standard) to evaluate the sensitivity and specificity of Phage assay. Phage assay results were available in 2 days whereas Proportion Methods results were obtain in 42 days. A total of 44 strains were detected as rifampicin resistant by both methods. However, one strains deemed resistant by Proportion Methods was susceptible by Phage assay. The sensitivity and specificity of Phage assay were 97,8 percent and 100 percent respectively. Phage assay provides rapid and reliable results for susceptibility testing; it's easy to perform, requires no specialized equipment and is applicable to drug susceptibility testing in low income countries where tuberculosis is a major public health problem(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Cuba
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Tuberculose
Base de dados:
CUMED
Assunto principal:
Rifampina
/
Farmacorresistência Bacteriana
/
Antibióticos Antituberculose
/
Micobacteriófagos
/
Mycobacterium tuberculosis
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Inglês
Revista:
Ann Clin Microbiol Antimicrob
Ano de publicação:
2006
Tipo de documento:
Artigo
Instituição/País de afiliação:
Institue of Tropical Medicine/Belgium
/
Instituto de Medicina Tropical Pedro Kouri/Cuba
/
London School of Hygiene and tropical Medicine/London