Improved pharmacological properties for superoxide dismutase modified with mannan
Biotechnol Appl Biochem
; 44(3): 159-165, June. 2006. graf
Artigo
em Inglês
| CUMED
| ID: cum-49488
Biblioteca responsável:
CU424.1
Localização: CU424.1
ABSTRACT
Mannan from Sacharomyces cerevisiae was activated by oxidation with NaIO4 (sodium m-periodate) and further linked to SOD (superoxide dismutase) via reductive alkylation with NaBH4 (sodium borohydride). The gly-cosidated enzyme contained an average of 1,2 mol of polysaccharide per mol of protein and retained 52 per cent of its initial activity. The modified enzyme was 560-fold more resistant to inactivation with H2O2 and acquired a lectin-recognition capacity in respect of concanavalin A. The anti-inflammatory activity of SOD was increased 2-fold and its plasma half-life time was prolonged from 4.8 min to 1.7 h after glycosylation with the polymer(AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Cuba
Base de dados:
CUMED
Assunto principal:
Superóxido Dismutase
/
Mananas
/
Anti-Inflamatórios
Limite:
Animais
Idioma:
Inglês
Revista:
Biotechnol Appl Biochem
Ano de publicação:
2006
Tipo de documento:
Artigo
Instituição/País de afiliação:
Universidad de Matanzas/Cuba