Toxicidad retiniana de doxiciclina intravitrea. Estudio piloto / Intravitreal toxicity of doxycycline. A pilot study
Arch. Soc. Esp. Oftalmol
; 82(4): 223-228, abr. 2007. ilus
Artigo
em Es
| IBECS
| ID: ibc-054905
Biblioteca responsável:
ES1.1
Localização: ES1.1 - BNCS
RESUMEN
Objetivo:
Valorar la toxicidad retiniana de la doxiciclina administrada en inyecciones intravítreas.Método:
Utilizamos catorce conejos albinos neozelandeses que dividimos en 5 grupos. Inyectamos en dos ojos cada una de las siguientes concentraciones de doxiciclina 2.000 µg/0,1 ml; 500 µg/0,1 ml; 250 µg/0,1 ml; 125 µg/0,1 ml y 67,5 µg/0,1 ml. Dos ojos, usados como control, recibieron 0,1 ml de solución de dextrosa al 5%. Examinamos a todos los animales antes y después de la inyección con oftalmoscopía indirecta y biomicroscopía. Realizamos electrorretinogramas en todos antes de la inyección intravítrea y dos semanas después; y posteriormente los animales fueron eutanizados. Se enuclearon los ojos y se prepararon para estudio histológico.Resultados:
Los grupos que mostraron una disminución significativa en los electrorretinogramas fueron los inyectados con las siguientes concentraciones de doxiciclina 2.000 µg/0,1 ml; 500 µg/0,1 ml y 250 µg/0,1 ml. No se observaron cambios significativos en los electrorretinogramas de los inyectados con 125 y 67,5 µg/0,1 ml. No hubo signos de toxicidad retiniana en los exámenes de biomicroscopía, en oftalmoscopía indirecta e histología de los inyectados con 125 µg/0,1 ml o niveles menores de doxiciclina.Conclusión:
Las inyecciones intravítreas de doxiciclina parecen seguras en un rango igual o menor a 125 µg/0,1 ml en el conejo albino. La doxiciclina intravítrea podría ser una alternativa barata y beneficiosa para el tratamiento de la endoftalmitis, especialmente frente a los staphylococcus aureus resistentesABSTRACT
Objective:
To assess the retinal toxicity of varying concentrations of intravitreally administered doxycycline, a member of tetracycline family.Methods:
Fourteen New Zealand albino rabbits, divided into 5 groups, were used for this study. The initial concentration of doxycycline (100 mg) was titrated using 5% dextrose solution to the following concentrations in a volume of 0.1 ml 2000 µg, 1000 µg, 500 µg, 250 µg, 125 µg, and 62.5 µg. Each concentration was injected into 2 rabbit eyes. Two control eyes received 0.1 ml of 5% dextrose solution. All animals were examined before and after injection using indirect ophthalmoscopy and slitlamp biomicroscopy. Electroretinography (ERG) was performed on all animals prior to the intravitreal injection and 2 weeks post-injection. The animals were re-examined at this time by indirect ophthalmoscopy and slitlamp biomicroscopy and were then subjected to euthanasia. Their eyes were enucleated and examined using light microscopy.Results:
The doxycycline injected group exhibited significant decreases in ERG of the eyes injected with 2000 µg, 1000 µg, 500 µg, and 250 µg/0.1 ml. No significant changes in the ERG were observed following the injection of lesser concentration levels. There were no signs of retinal toxicity on slit-lamp examination, indirect ophthalmoscopy, or light microscopy in all the eyes injected with doxycycline concentrations of 125 µg or lower.Conclusions:
Doxycycline injected intravitreally appeared safe at concentrations of 125 µg/0.1 ml or less in albino rabbits. Intravitreal doxycycline may be beneficial, and is an inexpensive alternative drug which could be used in the treatment of bacterial endophthalmitis particularly against resistant Staphylococcus aureus organisms
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Oftalmoscopia
/
Endoftalmite
/
Doxiciclina
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Espanhol
Revista:
Arch. Soc. Esp. Oftalmol
Ano de publicação:
2007
Tipo de documento:
Artigo
Instituição/País de afiliação:
HHUU Virgen del Rocío/España
/
Tulane University Health Sciences Center/USA