A novel peptide (Thx) homing to non-small cell lung cancer identified by ex vivo phage display
Clin. transl. oncol. (Print)
; 15(6): 492-498, jun. 2013. tab, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-127393
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
AIM:
To identify linear peptide homing to non-small cell lung cancer (NSCLC) tumor cells using ex vivo phage display method. MATERIALS ANDMETHODS:
Twenty-six clinical patient samples were used to identify linear homing peptide, which was exposed to NSCLC cell cultures and control cell lines to determine cell binding affinity and cell localization. Also, ex vivo biodistribution was analyzed using tumor-bearing mice.RESULTS:
The panning yielded peptide enrichment with a core motif (A)/SRXPXXX. Based on this, an amino acid sequence, ARRPKLD, was selected for characterization and named Thx-peptide. The in vitro binding properties of Thx-peptide demonstrated selectivity toward NSCLC. Internalization assays showed that Thx-Alexa and fluorescein conjugates were located in a subset of perinuclearly located lysosomes of tumor cells. Thx-peptide appeared with fluorescein-labeled peptide and peptide-DTPA-chelator complex in adenocarcinoma xenografts in mice.CONCLUSION:
Thx shows promise for targeted imaging and drug delivery (AU)
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Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Neoplasias Pulmonares
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2013
Tipo de documento:
Artigo
Instituição/País de afiliação:
Helsinki University Central Hospital/Finland
/
Karyon-CTT/Finland