Deregulation of NF-kBmiR-146a negative feedback loop may be involved in the pathogenesis of diabetic neuropathy
J. physiol. biochem
; 71(1): 51-58, mar. 2015.
Artigo
em Inglês
| IBECS
| ID: ibc-133902
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
The current study was designed to explore whether microRNA-146a and its adapter proteins (tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6) and interleukin-1 receptor-associated kinase 1 (IRAK1)) are involved in the pathogenesis of diabetes neuropathy. Twelve male Sprague Dawley rats were randomized into control and diabetic groups (n = 6). Diabetes was induced by a single-dose injection of nicotinamide (110 mg/kg; i.p.), 15 min before injection of streptozotocin (50 mg/kg; i.p.) in 12-h-fasted rats. Diabetic neuropathy was evaluated by hot plate and tail emersion tests, 2 months after the injection of streptozotocin. The gene expression level of microRNA-146a (miR-146a), IRAK1, TRAF6, and nuclear factor kappa B (NF-κB) was measured in the sciatic nerve of rats using the real time-PCR method. Moreover, the activity of NF-κB and the concentration of pro-inflammatory cytokines were determined by the ELISA method. In comparison with the control group, a threefold increase in the expression of miR-146a and NF-κB, and a twofold decrease in the expression of TRAF6 were observed in the sciatic nerve of diabetic rats. Furthermore, the NF-κB activity and the concentration of TNF-α, interleukin 6 (IL-6), and interleukin 1β (IL-1β) in the sciatic nerve of diabetic rats were higher than in those of control counterparts. These results suggest that a defect in the NF-кBmiR-146a negative feedback loop may be involved in the pathogenesis of diabetic neuropathy
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Base de dados:
IBECS
Assunto principal:
Nervo Isquiático
/
MicroRNAs
/
Neuropatias Diabéticas
Tipo de estudo:
Estudo de etiologia
/
Estudo de avaliação
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Estudo observacional
/
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
J. physiol. biochem
Ano de publicação:
2015
Tipo de documento:
Artigo
Instituição/País de afiliação:
Hormozgan University of Medical Sciences/Iran
/
Tabriz University of Medical Sciences/Iran