Propuesta de un panel MIRU-VNTR 5 para el tamizaje de aislamientos clínicos de Mycobacterium tuberculosis en México / Proposal of a five MIRU-VNTR panel to screen clinical isolates of Mycobacterium tuberculosis in Mexico

RESUMEN

INTRODUCCIÓN: La tuberculosis es un problema de salud pública que se distribuye de forma diferenciada en regiones de México. El presente trabajo propone un panel, con el mínimo número de MIRU-VNTR (mycobacterial interspersed repetitive unit-variable number tandem repeats), para la diferenciación preliminar de aislamientos clínicos de Mycobacterium tuberculosis (M. tuberculosis). MÉTODO: Se realizó el panel completo de 24 MIRU-VNTR a 65 aislamientos clínicos de M. tuberculosis provenientes de diferentes regiones geográficas de México. RESULTADOS: Se presenta un panel de cinco loci MIRU-VNTR para discriminar aislamientos clínicos de M. tuberculosis de tres diferentes regiones geográficas de México. CONCLUSIONES: La utilización del panel MIRU-VNTR 5 podría utilizarse como tamizaje durante la caracterización genotípica de aislamientos clínicos de M. tuberculosis en México

ABSTRACT

INTRODUCTION: Tuberculosis is a public health problem across Mexico. This paper aims to select a panel, with a minimum number of repetitive elements (MIRU-VNTR) for genotypic characterization of Mycobacterium tuberculosis (M. tuberculosis) clinical isolates. METHOD: In this study, a full panel of 24 MIRU-VNTR loci was used to discriminate 65 clinical isolates of M. tuberculosis from three different geographical regions of Mexico. Those loci with the highest discriminatory power were subsequently selected. RESULTS: The panel, including five loci, was obtained by selecting the highest values of allelic diversity among the genotypes obtained. The dendrogram, generated by the panel MIRU-VNTR 5, showed a high discriminatory power with 65 unique genotype profiles and formed clusters according to the geographical region of origin. CONCLUSIONS: The panel MIRU-VNTR 5 can be useful for characterizing clinical isolates of M. tuberculosis in Mexico