Genetic and serologic surveillance of rotavirus with P[8] and P[4] genotypes in feces from children in the city of Chihuahua, northern Mexico
Int. microbiol
; 19(1): 27-32, mar. 2016. ilus, tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-157081
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Rotavirus vaccine was developed using the most prominent G and P genotypes circulating in children population. Therefore, severe gastroenteritis has been reduced around the world. This study investigated the G and P rotavirus genotypes circulating in children from two hospitals in the city of Chihuahua, Mexico. Additionally, polyclonal antibodies against Rotavirus Wa strain were used to determine their homotypic and heterotypic reactivity to both P[8] and P[4] genotypes. G1, G2, and G3 VP7 genotypes and P[8] and P[4] VP4 genotypes were detected in common and uncommon combinations as well as mixed infectious. The predominant combination was G1P[8]. Phylogenetic analysis of VP4 gene revealed the presence of P[8]-1 and P[8]-3 lineages of P[8] genotype and P[4]-5 lineage of P[4] genotype. All but five G1P[8] rotavirus were detected by polyclonal anti-Rotavirus Wa strain. Mutation analysis revealed differences in three of the four neutralizing epitopes previously reported to VP8* subunit of VP4 protein. Results of this study offer insights over genetic variants of field rotavirus that could be detected in a homotypic and heterotypic way by antibodies elicited to rotavirus with P[8] genotype (AU)
RESUMEN
No disponible
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
/
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
/
Diarreia
Base de dados:
IBECS
Assunto principal:
Rotavirus
/
Mapeamento de Epitopos
/
Fezes
/
Técnicas de Genotipagem
Tipo de estudo:
Estudo de rastreamento
Limite:
Criança
/
Humanos
País/Região como assunto:
México
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2016
Tipo de documento:
Artigo
Instituição/País de afiliação:
Autonomous University of Chihuahua/Mexico
/
Autonomous University of Nuevo Leon/Mexico