Metabolic characterization of two different non-alcoholic fatty liver disease pre-clinical mouse models
Rev. esp. enferm. dig
; 111(4): 301-307, abr. 2019. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-189927
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
Introduction:
non-alcoholic fatty liver disease is one of the most prevalent liver disorders in the developed world. Currently, there is no approved pharmacological therapy except for lifestyle intervention. Therefore, there is a need to increase the knowledge of preclinical models in order to boost novel discoveries that could lead to a better therapeutic management. Material andmethods:
this study characterized the effects of two different diets, a long-term high-fat high-fructose diet (HF-HFD) and a choline-deficient, methionine supplemented high-fat diet (CDA-HFD) in C57BL/6J mice for 52 weeks or 16 weeks, respectively. Body weight, lipid and hepatic profile were analyzed and liver histology was subsequently evaluated.Results:
HF-HFD animals had an increased body weight and total cholesterol levels, whereas the opposite occurred in CDA-HFD. Both HF-HFD and CDA-HFD animals had higher ALT and AST levels. With regard to histology findings, HF-HFD and CDA-HFD diets induced an increased collagen deposit and intrahepatic steatosis accumulation.Conclusion:
in conclusion, the comparison of these models aided in the selection of a long-term, more physiological model for physiopathology studies or a more rapid NASH model for novel molecule testingRESUMEN
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Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Fígado Gorduroso
/
Hepatopatia Gordurosa não Alcoólica
/
Cirrose Hepática
Limite:
Animais
Idioma:
Inglês
Revista:
Rev. esp. enferm. dig
Ano de publicação:
2019
Tipo de documento:
Artigo
Instituição/País de afiliação:
Centro Andaluz de Biología Molecular y Medicina Regenerativa (CABIMER)/Spain
/
Universidad de Sevilla/Spain