First identification of blaNDM-1 carbapenemase in blaOXA-94-producing Acinetobacter baumannii ST85 in Spain / Detección por primera vez en España de la carbapenemasa blaNDM-1 en Acinetobacter baumannii ST85 productor de blaOXA-94
Enferm. infecc. microbiol. clín. (Ed. impr.)
; 38(1): 11-15, ene. 2020. tab, graf
Artigo
em Inglês
| IBECS
| ID: ibc-200427
Biblioteca responsável:
ES1.1
Localização: BNCS
ABSTRACT
INTRODUCTION:
NDM-1 carbapenemase is spreading rapidly all over the world, but this metallo-beta-lactamase has just been detected for the first time in an Acinetobacter baumannii (Ab) isolate of the ST85 clone in Spain. The aim of this study was to characterize a NDM-1-producing carbapenem-resistant A. baumannii (CR-Ab) isolate submitted to the Andalusian PIRASOA [infection prevention program] referral laboratory.METHODS:
Carbapenemases were detected by PCR and Sanger DNA sequencing. Whole genome sequencing was performed by NGS (Miseq, Illumina). Resistance genes were identified with RESfinder, while MLSTfinder was used for sequence typing (ST). The genetic location of blaNDM-1 was determined by nuclease S-1/PFGE/hybridization with specific probe.RESULTS:
The isolate was susceptible to amikacin and tigecycline and belonged to the ST85 clone. blaOXA-94 and blaNDM-1 were identified by PCR and Sanger DNA sequencing, respectively. The resistance genes aadB, blaADC-25, blaNDM-1, blaOXA-94, msr(E), mph(E) and floR,sul2 were identified by NGS. The chromosome of the isolate contained a defective Tn125 transposon with blaNDM-1 flanked by the insertion sequences ISAbA125 and ISAba14. The blaNDM-1 gene was only detected in the chromosomal DNA.CONCLUSION:
This is the first time that blaNDM-1 has been detected and characterized in a blaOXA-94-producing CR-Ab isolate belonging to the ST85 clone in Spain
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Resistência beta-Lactâmica
/
Acinetobacter baumannii
/
Beta-Lactamas
/
Antibacterianos
Limite:
Humanos
País/Região como assunto:
Europa
Idioma:
Inglês
Revista:
Enferm. infecc. microbiol. clín. (Ed. impr.)
Ano de publicação:
2020
Tipo de documento:
Artigo
Instituição/País de afiliação:
Hospital Universitario Puerta del Mar/Spain
/
Hospital Universitario Virgen Macarena/Spain
/
Centro Superior de Investigaciones Científicas (CSIC)
/
Instituto de Biomedicina de Sevilla (IBiS)/Spain
/
0016