MiR-181a promotes cell proliferation and migration through targeting KLF15 in papillary thyroid cancer
Clin. transl. oncol. (Print)
; 24(1): 66-75, enero 2022.
Artigo
em Inglês
| IBECS
| ID: ibc-203415
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
IntroductionPapillary thyroid cancer (PTC) is the predominant histological type of thyroid cancer, accounting for 80% of thyroid cancers. MiR-181a is a novel microRNA that is usually upregulated in multiple cancers. This study aims to explore the role and underlying mechanism of miR-181a in PTC.MethodsCCK8 and Transwell assays were performed to evaluate cell viability and migration. The mRNA level of miR-181a and KLF15 was calculated by qRT-PCR. The protein level of E-Cadherin, N-Cadherin and GAPDH was evaluated by western blot. Dual luciferase assay was conducted to validate that miR-181a directly targeting the 3′-UTR of KLF15 mRNA in TPC-1 cells.ResultsWe observed that miR-181a was overexpressed and KLF15 was low expressed in PTC tissues and cell lines. Upregulation of miR-181a or downregulation of KLF15 predicted poor outcomes in PTC patients. MiR-181a improved cell growth of PTC, migration and epithelialmesenchymal transition (EMT) in TPC-1 cells. KLF15 was a target gene of miR-181a and its expression was mediated by miR-181a. KLF15 partially reversed the facilitating effect of miR-181a on cell proliferation and migration in TPC-1 cells.ConclusionWe discovered that miR-181a served as an oncogene downregulating KLF15, thereby inhibiting cell proliferation, migration and the EMT. These findings demonstrate that miR-181a plays a significant role in PTC progression and could be a therapeutic target for PTC.
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Espanha
Base de dados:
IBECS
Assunto principal:
Neoplasias da Glândula Tireoide
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MicroRNAs
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Ciências da Saúde
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Câncer Papilífero da Tireoide
Limite:
Humanos
Idioma:
Inglês
Revista:
Clin. transl. oncol. (Print)
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Jinan Central Hospital/China
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Qingdao Hospital of Traditional Chinese Medicine/China
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Rizhao Hospital of TCM/China
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Yantaishan Hospital/China
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Zhangqiu District Peoples Hospital/China