Cinnamtannin D1 ameliorates DSS-induced colitis by preventing Th17/Treg imbalance through activation of the AMPK/mTOR pathway
Allergol. immunopatol
; 50(5): 153-161, sept. 2022. graf, ilus
Artigo
em Inglês
| IBECS
| ID: ibc-208634
Biblioteca responsável:
ES1.1
Localização: E15.1 - BNCS
ABSTRACT
Background:
Inflammatory bowel disease (IBD) is a chronic idiopathic gastrointestinal dis-ease, including ulcerative colitis (UC) and Crohns disease (CD), which is typically charac-terized by chronicity and relapse. Cinnamtannin D1 (CTD1), extracted from Cinnamomum tamala, has been found to exert good immunosuppressive activity. However, the role of CTD1 in IBD is unclear.Methods:
The colitis mice model was established by dextran sulfate sodium (DSS) treat-ment. Protein levels (p-STAT3/STAT3, ROR-γt, p-STAT5/STAT5, FOXP3, p-AMPK/AMPK, and p-mTOR/mTOR) were examined using Western blotting analysis. Changes in histopathol-ogy were detected through hematoxylin and eosin staining. The proportion of T helper 17 (Th17) cells and regulatory T (Treg) cells was measured by flow cytometry analysis.Results:
CTD1 improved body weight and colon length, and alleviated inflammation and histological damage in DSS-induced colitis mice model. DSS treatment also demonstrated a negative effect on Th17Treg cells balance whereas CTD1 restored the balance of Th17Treg cells in DSS-induced colitis mice model. Regulatory factors (such as STAT3, ROR-γt, STAT5, and FOXP3) that closely related to the balance of Th17Treg cells were regulated by CTD1. In addition, AMPK phosphorylation was increased and mTOR phosphorylation was inhibited by CTD1 in DSS-induced colitis mice model.Conclusion:
These findings established that CTD1 improved DSS-induced colitis by suppress-ing Th17Treg cells balance by activating the AMPK/mTOR pathway. This study provided a new strategy for developing novel treatments for patients with IBD (AU)
Texto completo:
Disponível
Coleções:
Bases de dados nacionais
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Espanha
Base de dados:
IBECS
Assunto principal:
Doenças Inflamatórias Intestinais
/
Colite
Limite:
Animais
Idioma:
Inglês
Revista:
Allergol. immunopatol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Chongqing Traditional Chinese Medicine Hospital/China
/
The Second Affiliated Hospital of Chongqing Medical University/China