Antimicrobial peptide AMP-17 exerts antiCandida albicans effects through ROS-mediated apoptosis and necrosis / Péptido antimicrobiano AMP-17 ejerce efectos albicans anti-Candida a través de ROS-mediada apoptosis y necrosis
Int. microbiol
; 26(1): 81-90, Ene. 2023. ilus
Article
em En
| IBECS
| ID: ibc-215919
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ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
There is a need for new antiCandida albicans (C. albicans) drugs owing to the emergence of drug resistance in recent years. AMP-17, an antimicrobial peptide from Musca domestica (M. domestica), is known to be an effective inhibitor of many fungal pathogens, including C. albicans. In this study, we investigated the potential mechanism underlying the antiC. albicans effects of AMP-17 using flow cytometry, transmission electron microscopy, fluorescent probes, fluorescence microplate reader, and confocal laser microscopy. Transmission electron microscopy showed that, following AMP-17 treatment, the shape of C. albicans cells became irregular, and vacuoles could be seen in the cytoplasm. Furthermore, AMP-17 treatment resulted in an increase in reactive oxygen species (ROS) levels, depolarization of the mitochondrial membrane potential (MMP), and changes in the cell cycle, leading to the apoptosis and necrosis, which ultimately contributed to the death of C. albicans cells.(AU)
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Coleções:
06-national
/
ES
Base de dados:
IBECS
Assunto principal:
Candida albicans
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Ciclo Celular
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Apoptose
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Microscopia Eletrônica de Transmissão
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Citometria de Fluxo
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Corantes Fluorescentes
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Necrose
Limite:
Humans
Idioma:
En
Revista:
Int. microbiol
Ano de publicação:
2023
Tipo de documento:
Article