Enhancement of vincristine under in vitro culture of Catharanthus roseus supplemented with Alternaria sesami endophytic fungal extract as a biotic elicitor / Mejora de vincristina bajo cultivo in vitro de Catharanthus roseus suplementado con extracto de hongos endófitos de Alternaria sésamo como un elicitor biótico
Int. microbiol
; 25(2): 275-284, May. 2022. ilus
Artigo
em Inglês
| IBECS
| ID: ibc-216031
Biblioteca responsável:
ES1.1
Localização: ES15.1 - BNCS
ABSTRACT
Vincristine, one of the major vinca alkaloid of Catharanthus roseus (L.) G. Don. (Apocynaceae), was enhanced under in vitro callus culture of C. roseus using fungal extract of an endophyte Alternaria sesami isolated from the surface-sterilized root cuttings of C. roseus. Vindoline, a precursor molecule for vincristine production, was detected for the first time in the fungal endophyte A. sesami which was used as a biotic elicitor in this study to enhance vincristine content in the C. roseus callus. It was identified using high-performance liquid chromatography and mass spectroscopy techniques by matching retention time and mass data with reference molecule. Supplementing the heat sterilized A. sesami endophytic fungal culture extract into the callus culture medium of C. roseus resulted in the enhancement of vincristine content in C. roseus callus by 21.717% after 105-day culture.(AU)
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Coleções:
Bases de dados nacionais
/
Espanha
Base de dados:
IBECS
Assunto principal:
Vincristina
/
Catharanthus
/
Alternaria
/
Endófitos
/
Fungos
Limite:
Humanos
Idioma:
Inglês
Revista:
Int. microbiol
Ano de publicação:
2022
Tipo de documento:
Artigo
Instituição/País de afiliação:
Jamia Hamdard/India